FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6f10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6f10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences967995
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA18270.18874064432150991No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC16200.167356236344196No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC16010.16539341628830728No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA14720.1520669011720102No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT13950.14411231462972432No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT12620.1303725742385033No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT11880.12272790665241039No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA11180.11549646434124143No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT10700.11053776104215415No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG10630.10981461681103726No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA10380.10723195884276263No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA10060.1039261566433711No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT9880.10206664290621337No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT9860.10186003026875139No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA28750.045.3958471
TTCCGAT30150.043.2834472
CGATCTG14300.042.0802845
GGGCGAC5700.041.973557
CCGATCT31150.041.6611824
GCGACCC7950.031.9181379
GTATTAA1703.009236E-629.8559281
TCCGATC46400.028.437413
GGCGACC8650.027.6588698
TAGAGTG6250.026.6799145
AAATTAG6250.025.5225541
TATTATA2301.1030861E-625.2173122
CTAGGGG1500.001494266224.166593
CGATCTA7000.023.8213525
CTTATAA5850.023.546932145
ATACAGT3702.6011548E-1023.5134376
CAGGGGC2802.342531E-723.3034974
ACCATAC8500.023.0317171
AGGTGCC8900.022.8089167
TATACAG3203.385503E-822.6561785
Produced by FastQC (version 0.11.2)