FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558030
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8890.1593104313388169No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5890.1055498808307797No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA82050.041.794641
CCGATCT80500.041.7888224
TTCCGAT80700.041.7750932
TCCGATC83600.040.3259543
ATCTAGC2900.040.07
CGATCTG29850.039.5896155
CGATCTT18800.039.3351065
TATATCG800.002035844636.2500043
CGATCTC27450.035.1275025
GATCTCG6950.034.424466
TGGACCG2404.3655746E-1133.2291685
GATCTAG6300.032.222226
ATCTGCC6850.031.7518237
GATCTTT11700.031.6025646
TATATGA1153.1603212E-431.521742
ACATTAT1453.4876597E-530.01
ATCTTGC3650.029.794527
ATCTCGC4750.029.07
ATCTTAC2054.0337545E-728.2926837
ATTATAT2054.0337545E-728.2926833
Produced by FastQC (version 0.11.2)