FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235900
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11880.5036032217041119No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7640.32386604493429416No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5390.22848664688427298No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG5190.22000847816871558No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4660.19754133107248834No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA4030.1708350996184824No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3960.16786774056803733No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT3790.16066129715981348No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC3560.15091140313692242No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.1466723187791437No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT3320.1407376006782535No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG3150.13353115727002968No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT2830.11996608732513776No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2710.1148791860958033No Hit
TTGTAGACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTT2540.1076727426875795Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG2440.10343365832980077No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA2430.1030097498940229No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC2390.1013141161509114No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA2370.10046629927935567No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA2370.10046629927935567No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT2360.10004239084357779No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAG350.003563127562.1428571
ATCTAGG502.0070828E-458.07
TTCTAGA805.854381E-754.3751
CATACCC400.00604517854.3754
CCATACA450.00963007848.3333324
GCTAAAC450.00963007848.333332145
CTAGGCC450.00963007848.3333329
TGTAGAC855.329043E-542.6470572
ATCTTAC700.001054998741.428577
CTAGGGG700.001054998741.428573
TTAGAGT1105.2472005E-639.5454524
CTAGACG2400.039.2708323
TCTAGGG750.001481087138.6666642
ACACACG1701.8171704E-938.382351
TCATAAT951.02650374E-438.1578942
CGATCTG19950.037.7944835
GTCATAA1209.532907E-636.251
AGCCTAC800.002033467436.251
ATCTGCG2451.8189894E-1235.5102047
CTAGGGA1854.152753E-935.2702673
Produced by FastQC (version 0.11.2)