FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183566
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24721.3466546092413627No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9950.5420393754834774No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7690.4189228942178835No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6210.3382979418846627No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4050.22062909253347568No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3350.18249566913262805No Hit
TTGTAGACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTT3050.16615277338940762Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TTCTAGACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTT2190.11930313892550909Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT2040.11113169105389886No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA203.8568198E-4108.7794341
TGTAGAC1100.065.9089662
GTCTAAG350.003558050462.1596681
GGTTACT350.003561894262.142747
TATATTG450.009626768548.3332445
ATATGGC450.009626768548.3332443
TAGGACC951.9122908E-645.7893874
TAGACGT4300.043.8371284
TCTAGAC1755.2750693E-1141.4284972
TATATGG700.001054395741.4284932
TTGTAGA1651.3496901E-939.5561561
TTAGGAC1105.240894E-639.545383
TTCTAGA1303.6686288E-739.0490261
CATTAGC750.001478261438.677131
TAATACT750.001480242938.6665954
TCTGTCC750.001480242938.6665958
GGCTCCT750.001480242938.6665957
CTAATCC750.001480242938.6665953
TACAGGG951.0256254E-438.157822
GTAGACG2300.037.826013
Produced by FastQC (version 0.11.2)