FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376864
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA6690.17751761908805297No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA5430.1440838074212448No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC5430.1440838074212448No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT4960.13161246497410206No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4680.12418272904814469No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT4290.11383416829413263No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA4110.10905790948458861No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT3920.10401630296340324No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA3840.10189352127027256No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACATA2050.042.438988145
TCTGTCC750.001482218738.6666348
TTCCGAT12200.036.844232
CCGATCT12550.035.239014
CTATAAG4550.035.0549132
ATAACGC1251.2630084E-534.799979
CTTCCGA12400.034.5005151
CCTATAA4600.033.102191
TATAAGC4950.032.2221953
CGATCTG5450.031.9265775
TGTACTG1153.1584065E-431.521715
CGATCTA3000.031.416645
ATAATTC3050.030.9016113
GGCGACC2252.8072463E-828.9999738
TATAATT3001.8189894E-1128.9999732
TAGACGT1358.073362E-426.8518284
TATACTG1358.073362E-426.8518285
AATGTAC1100.00966412826.3636113
GGGCGAC2808.1618055E-925.8928347
AATATAT1701.02962906E-425.5882135
Produced by FastQC (version 0.11.2)