FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345520
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11570.334857605927298No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7350.21272285251215556No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5240.1516554758045844No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG4880.1412363973141931No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT4170.12068765918036584No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4030.11663579532299143No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3770.10911090530215327No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.10534846029173421No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA800.002034773836.254
AAGCACA1406.592945E-736.251
ACACATA2302.5465852E-1134.673916145
ATCTCTC2103.765308E-1034.523817
CGATCTT16100.034.2236065
TCTAACG850.00273951434.1176452
ACACTGT850.00273951434.1176456
ATCTGTG2154.838512E-1033.7209327
TTCCGAT58350.033.5475582
CCGATCT58250.033.231764
TGTAGAC1102.4328665E-432.9545482
CTTCCGA60100.032.6913451
ATCTTAC1801.2882447E-732.222227
TCCGATC61650.031.5166283
CGATCTG23750.031.4421065
GATCTTA2558.731149E-1131.274516
TATCAGT1204.0526045E-430.2083342
TATAGGT2401.5734258E-930.2083343
CTAGACG2451.9626896E-929.5918373
GATCTCT3700.029.391896