FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6e12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6e12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608312
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG35130.5774997040992123No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC23800.3912465971409408No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC22750.3739857178553111No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA21190.3483409829166612No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT19240.3162850642433488No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG18240.2998461315903681No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA16580.27255750338642015No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA15490.2546390667946712No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC14850.2441181498967635No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG14610.24017280606004812No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA14610.24017280606004812No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC13190.21682952169281552No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT12410.20400715422349058No Hit
ACCTCCGGCTGAGCCGTTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGA11270.18526677099909258No Hit
ATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGG10980.18049948052972817No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10880.1788555872644301No Hit
GATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAG10430.1714580675705888No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA10390.17080051026446955No Hit
ATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAAC10370.17047173161140994No Hit
ATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGCGCGAACTTC10360.17030734228488015No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT10200.16767711306040323No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT9880.16241665461144938No Hit
ACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGG9860.16208787595838978No Hit
GATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAA9800.16110153999921092No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA9770.1606083720196215No Hit
ATTTTAACCGGATCCCCTTTCGAAGTTCGCGCTTAAGCGCTATCAGACGG9360.15386840963189943No Hit
CTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAA9300.15288207367272058No Hit
GTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGACGTCTCCGGA9200.1512381804074225No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG9050.1487723405094754No Hit
GGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGC8600.14137482081563407No Hit
GCACATGGGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCG8470.1392377595707466No Hit
AGATAACTCTTCCCGAGGCCCCCGCCGACGTCTCCGGACTCCCTAACGTT8090.1329909651626139No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG8090.1329909651626139No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT8060.13249779718302448No Hit
CTGTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGACGTCTCCG7760.1275661173871303No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT7640.1255934454687726No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG7620.125264666815713No Hit
GTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATG7610.12510027748918318No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT7580.12460710950959378No Hit
CTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTT7530.12378516287694472No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG7430.12214126961164666No Hit
CTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACC7290.11983981904022936No Hit
AACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCT7250.11918226173411013No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCT7110.11688081116269283No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG7060.1160588645300438No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC6970.11457936059127553No Hit
GATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAA6890.11326424597903709No Hit
AGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATA6830.11227791001985822No Hit
ATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGT6780.1114559633872092No Hit
GTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTC6730.11063401675456017No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGG6680.10981207012191112No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG6600.10849695550967267No Hit
GGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAAGAGTCG6590.10833256618314287No Hit
CTTAAGCGCTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCC6540.10751061955049383No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA6450.10603111561172555No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT6420.10553794763213614No Hit
GTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTT6340.10422283301989768No Hit
GCACTTGCACATGGGTTAGTCGATCCTAAGAGTCGGGGGAAACCCGTCTG6320.10389405436683806No Hit
AATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCAC6270.10307210773418904No Hit
CTCTTCCCGAGGCCCCCGCCGACGTCTCCGGACTCCCTAACGTTGCCGTC6220.10225016110154No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6150.10109943581583135No Hit
CTGCAAAACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATC6110.10044187850971212No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGACT451.19349366E-464.4443364
TCTGAGC1600.058.9061478
ATCTGAG2600.055.7691357
TTAGAGT3950.055.06324
AAATTAG4500.049.956681
CTTCCGA81800.046.7198751
TTCCGAT82250.046.3646622
TACAGGA2700.045.648072
TAGAGTG4950.045.4039655
CCGATCT83750.045.361124
CGATCTT20350.043.820565
CGATCTG35200.043.4587335
GATCTGA10500.042.8094526
GTACAGG3250.042.3951
ATCTGCT2600.041.826857
TCTAGCA907.4790536E-540.277718
GTGCTCA5400.040.277719
ATCTTAC1807.2759576E-1140.277717
TCCGATC95050.040.1971973
AATTAGA5550.039.189122
Produced by FastQC (version 0.11.2)