FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6e11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6e11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366704
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27080.7384702648457612No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9050.24679305379815875No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7530.20534272874034643No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7410.20207033465683497No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG4950.13498625594484925No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4920.13416815742397137No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA4740.12925956629870414Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG3920.10689820672804223No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3830.1044439111654086No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTAGA1250.069.618861
GTACTAT400.006041364754.389731
CATAGTA450.00963437848.333248
ATCTAGC2800.041.4284937
ATCTATC1905.4060365E-934.342047
TAACACA850.002739757334.1175844
TCTGACC3350.032.4626248
CTAAGCG900.00362529232.222164
GATCTTT14150.031.7667266
ATCTTTT13550.031.5682057
CTGACCT1851.6388367E-731.351299
TCTTTTT13250.031.188628
CTAGACG6100.029.7130583
CGATCTT26050.029.5009065
CTATCCA1000.006069564328.9999479
CTTTTTT14200.028.5914979
TCTAGAC6350.028.5432552
TCTAGCC3102.7284841E-1128.0644658
CCCCTAG1050.00769201527.6265281
CTTGCTC1050.007702261727.6189963