Basic Statistics
Measure | Value |
---|---|
Filename | H55FTAFXY_n01_p6e11.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 366704 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2708 | 0.7384702648457612 | No Hit |
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 905 | 0.24679305379815875 | No Hit |
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 753 | 0.20534272874034643 | No Hit |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 741 | 0.20207033465683497 | No Hit |
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG | 495 | 0.13498625594484925 | No Hit |
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 492 | 0.13416815742397137 | No Hit |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 474 | 0.12925956629870414 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG | 392 | 0.10689820672804223 | No Hit |
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 383 | 0.1044439111654086 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TTCTAGA | 125 | 0.0 | 69.61886 | 1 |
GTACTAT | 40 | 0.0060413647 | 54.38973 | 1 |
CATAGTA | 45 | 0.009634378 | 48.33324 | 8 |
ATCTAGC | 280 | 0.0 | 41.428493 | 7 |
ATCTATC | 190 | 5.4060365E-9 | 34.34204 | 7 |
TAACACA | 85 | 0.0027397573 | 34.117584 | 4 |
TCTGACC | 335 | 0.0 | 32.462624 | 8 |
CTAAGCG | 90 | 0.003625292 | 32.22216 | 4 |
GATCTTT | 1415 | 0.0 | 31.766726 | 6 |
ATCTTTT | 1355 | 0.0 | 31.568205 | 7 |
CTGACCT | 185 | 1.6388367E-7 | 31.35129 | 9 |
TCTTTTT | 1325 | 0.0 | 31.18862 | 8 |
CTAGACG | 610 | 0.0 | 29.713058 | 3 |
CGATCTT | 2605 | 0.0 | 29.500906 | 5 |
CTATCCA | 100 | 0.0060695643 | 28.999947 | 9 |
CTTTTTT | 1420 | 0.0 | 28.591497 | 9 |
TCTAGAC | 635 | 0.0 | 28.543255 | 2 |
TCTAGCC | 310 | 2.7284841E-11 | 28.064465 | 8 |
CCCCTAG | 105 | 0.007692015 | 27.626528 | 1 |
CTTGCTC | 105 | 0.0077022617 | 27.618996 | 3 |