FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6e10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6e10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2331651
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA46690.20024437619523675No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC43070.1847188966101702No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC42350.18163095591921774No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA39320.16863587217812614No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT37860.16237421466591698No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT31290.13419675586097576No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT30840.13226679292913046No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA30120.12917885223817802No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT28780.12343185150779426No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG28670.12296008279112096No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT27450.11772773884256263No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA27300.11708441786528086No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC27020.11588355204102158No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT25710.11026521550609417No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA25700.11022232744094206No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA25460.10919301387729125No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC25260.10833525257424889No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT23960.10275980410447362No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC23510.10082984117262832No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT72350.051.4063342
CTTCCGA73850.050.2672581
CCGATCT74300.049.3741384
CGATCTG36300.044.338825
TCCGATC111450.033.241353
GGGCGAC16350.031.0397427
CGATCTA17100.029.6783495
TCGCGCG14250.027.4736739
CCATACT18900.027.235442
GTCGCGC14600.026.8150588
CATACTC21900.026.4840073
CGATCTC36150.026.2724655
CAGGGGC6900.026.2681054
CATACTA6450.025.8527012
CTTATAA15350.025.504875145
ACCATAC20350.024.9401681
ATATAGG4101.8189894E-1224.7576791
TCCCCCC19400.024.6649388
ATACTCC20350.024.58234
TATATAC8100.024.1666563
Produced by FastQC (version 0.11.2)