FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2030296
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG108950.5366212611363073No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG83320.41038351058170824No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT81500.4014193004369806No Hit
GATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAA80520.3965924180513581No Hit
TCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGT77100.3797475836035731No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG76800.3782699665467498No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG66200.32606083053899526No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGG64610.31822946013783215No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT63900.3147324331033504No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT62620.30842793366090465No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCT62610.3083786797590105No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC62400.30734434781923425No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT60350.29724729793094207No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA59890.2949816184438131No Hit
ATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAATGCCTCTAA57870.2850323302612033No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT57040.28094425640399234No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC56070.2761666279202638No Hit
CCTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTA55190.2718322845535823No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC54710.2694680972626652No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGT54340.26764570289258316No Hit
GGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTG52440.2582874615327026No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA51770.2549874501057974No Hit
GTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGA50330.2478948882330458No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT50120.24686055629326958No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC48280.23779783834475365No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCT48270.23774858444285957No Hit
TTTCAAGACGGGTCGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGC46980.2313948310985196No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTT46760.23031124525684926No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC46630.22967094453222583No Hit
CCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGAC46540.22922765941517886No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG46360.22834108918108492No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA45910.22612466359585004No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTA45530.22425301532387393No Hit
TCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTT44430.21883508611552208No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC43480.21415596543558182No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC43280.21317088739769965No Hit
GTCTTGAAACACGGACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTG40470.19933054096545527No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA40450.1992320331616671No Hit
ATACTGACGTGCAAATCGTTCGTCTGACTTGGGTATAGGGGCGAAAGACT40180.19790217781052613No Hit
AGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGC40010.1970648614783263No Hit
ATGGTGAACTATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAA39070.19243499470028017No Hit
GATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGT38660.1904155847226217No Hit
CCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTT38440.18933199888095134No Hit
CTCCACAAGCGTATCAAAAGCCCGGGCTTAGGCCGCCACCGTAATCCGCG38140.1878543818241281No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA38020.1872633350013988No Hit
TCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAG37380.1841110852801759No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCG37170.1830767533403996No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT36310.17884091777750633No Hit
GTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGA35560.17514687513544822No Hit
GTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAA35550.17509762123355413No Hit
GGCTTAGGCCGCCACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCG35440.17455582831271893No Hit
ATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCG35270.1737185119805191No Hit
TATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAA34620.17051700835740208No Hit
CGTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCA34530.1700737232403551No Hit
GGCTTTACCCCGCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGC33920.1670692352248145No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG33890.1669214735191322No Hit
TCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGA33760.16628117279450877No Hit
TTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCC33640.16569012597177948No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGAC33580.16539460256041486No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC33190.16347370038654463No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT33100.16303041526949763No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAAGGCTAAAT32980.16243936844676835No Hit
AACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGG32650.1608139896842628No Hit
CCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGG32550.16032145066532172No Hit
GATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTTGCAG32450.1598289116463806No Hit
CTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAA31560.155445314377805No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCC31440.15485426755507573No Hit
CATGTCAGACTCCTTGGTCCGTGTTTCAAGACGGGTCGAATGGGGAGCCC31410.15470650584939338No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA31230.15381993561529944No Hit
TAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCT31070.1530318731849937No Hit
TGTCAGACTCCTTGGTCCGTGTTTCAAGACGGGTCGAATGGGGAGCCCAC31060.1529826192830996No Hit
ACTTTAAATAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGA30510.15027365467892367No Hit
GTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGG30500.15022440077702956No Hit
AACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAA30320.14933783054293562No Hit
CCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTC30320.14933783054293562No Hit
ACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGT30200.14874678372020633No Hit
GCGCTTAAGCGCGCGACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCT29980.14766319787853593No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA29970.14761394397664185No Hit
CCGTTAGGCGCGTGCTGCAGACCACGATCACGGCAGCGACGTCTCCACAA29870.14712140495770074No Hit
TCTCGATTCCGTGTGACGGCTCAACAAAGCAGCCGACACGTCCTACCTAT29540.1454960261951952No Hit
GAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGC29480.14520050278383054No Hit
ACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAGGGAAACTTCGG29470.14515124888193642No Hit
CTTCTCAGAAGATCAAGGTCGGTCGGCGGTGCACCCGCGAGGGATCCCGC29370.14465870986299534No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT29030.14298407719859565No Hit
ATTCTCAAACTTTAAATAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGT28940.1425407920815487No Hit
AACTATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGC28800.14185123745503117No Hit
TCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAAT28640.14106317502472546No Hit
ACCTTGATCTTCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCC28460.14017660479063151No Hit
CCCAATGCCTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCT28290.1393392884584317No Hit
GGATAGCTGGAGCTCGGACGCGAGTTCTATCGGGTAAAGCCAATGATTAG28190.13884674943949057No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGT28180.1387974955375965No Hit
GTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATC28030.13805868700918486No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG27980.13781241749971432No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATT27900.13741838628456146No Hit
CCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCG27890.13736913238266735No Hit
GTTCACCATCTTTCGGGTCCCGACAGGCATGCTCACACTCGAACCCTTCT27670.13628554654099698No Hit
GAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG27070.1333303124273505No Hit
ACTTGGAGCTCTCGATTCCGTGTGACGGCTCAACAAAGCAGCCGACACGT26890.13244374219325655No Hit
CCCTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGT26840.132197472683786No Hit
GGCATAGTTCACCATCTTTCGGGTCCCGACAGGCATGCTCACACTCGAAC26410.13007955490233936No Hit
ACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCTTCCAC26160.12884820735498667No Hit
CCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAA26110.12860193784551613No Hit
CTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTT26030.12820790663036324No Hit
ACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGGCGGACCGGATTG25760.12687805127922233No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG25740.12677954347543413No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGT25730.12673028957354002No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAG25680.12648402006406947No Hit
CACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAAGTC25630.12623775055459893No Hit
GAATAGGTCGAGGGCGTTGCGCCCCCAATGCCTCTAATCATTGGCTTTAC25480.12549894202618733No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC25390.12505565690914033No Hit
CTTTAAATAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAA25210.12416908667504639No Hit
CCTAACGGCGTGCCTCGGCATCAGCGTGCTCCGGGCGTCGGCCTGTGGGC24700.1216571376784469No Hit
TCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCGG24570.12101683695382348No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG24250.11944071209321203No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG24180.11909593477995328No Hit
TAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAG24140.11889891917237683No Hit
TCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGC23820.11732279431176539No Hit
ATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGG23810.11727354040987127No Hit
GTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTT23800.11722428650797717No Hit
CATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTG23720.11683025529282429No Hit
GTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATT23720.11683025529282429No Hit
CCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGGTGCACCG23630.11638697017577733No Hit
GTTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGC23610.11628846237198912No Hit
ATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGCGCG23600.116239208470095No Hit
GACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAA23470.11559890774547159No Hit
CCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAA23360.11505711482463642No Hit
GAACTATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCG23120.11387502117917782No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT23120.11387502117917782No Hit
CGTCAGTATCGCTGCGGGCTTCCACCAGAGTTTCCTCTGGCTTTACCCCG23010.11333322825834262No Hit
AGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGTGAACTATGCCTGAGC22950.11303770484697798No Hit
ATTTTAACCGGATCCCCTTTCGAAGTTCGCGCTTAAGCGCTATCAGACGG22630.11146157998636651No Hit
GACTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTC22370.11018097853711971No Hit
ACCTCCGGCTGAGCCGTTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGA22270.10968843951817864No Hit
TGCCTGTCGGGACCCGAAAGATGGTGAACTATGCCTGAGCGGGGTAAAGC22210.10939291610681397No Hit
CGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCG22150.10909739269544932No Hit
ATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAAC22070.10870336148029647No Hit
CCTTGGTCCGTGTTTCAAGACGGGTCGAATGGGGAGCCCACAGGCCGACG21840.10757052173673198No Hit
GAACTGGCGATGCGGGATGAACCGGAAGCCGGGTTACGGTGCCCAACTGC21720.1069794749140027No Hit
GTATCGCTGCGGGCTTCCACCAGAGTTTCCTCTGGCTTTACCCCGCTCAG21720.1069794749140027No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA21690.10683171320832036No Hit
GTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAGAGTGCTTGAAATTG21660.10668395150263804No Hit
GGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCG21530.10604365077801464No Hit
TTGTTGAGCCGTCACACGGAATCGAGAGCTCCAAGTGGGCCATTTTTGGT21480.10579738126854409No Hit
GATCAAGGTCGGTCGGCGGTGCACCCGCGAGGGATCCCGCCAATCAGCTT21480.10579738126854409No Hit
GTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAA21200.10441827201550907No Hit
GATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAG21050.10367946348709746No Hit
GTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAATGCCTCTAATCATTGG21000.1034331939776269No Hit
ATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCTTCC20900.10294065495868583No Hit
ACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTT20800.10244811593974476No Hit
ACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGG20670.10180781521512135No Hit
GGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAGGGAAA20640.10166005350943902No Hit
ATCCTAAGAGTCGGGGGAAACCCGTCTGATAGCGCTTAAGCGCGAACTTC20370.10033019815829809No Hit
GGTCTGCAGCACGCGCCTAACGGCGTGCCTCGGCATCAGCGTGCTCCGGG20340.10018243645261579No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGTG2300.078.80429145
CCTACCA4100.067.19507145
CGATTAT2350.061.702087145
TTCCGAT77600.060.8214762
CTTCCGA78100.060.4380531
CCGATCT78950.059.965134
CTAAACT502.010747E-457.9999584
CGATCTG31000.057.532225
ATAAATA6500.053.538425145
ATCTCGC4500.053.1666347
CGAGGGG7350.052.278873145
CGATCTC27450.050.7103465
CGATCTT17550.049.985725
TAGAGTG10150.049.2856835
TCCGATC98600.048.1617283
CGATCTA14650.048.0033845
ATCTGCC6550.047.5953877
GGCTGCG4000.047.124966145
CTTATAA7750.046.774162145
GGCGACC21100.046.7298288
Produced by FastQC (version 0.11.2)