Basic Statistics
Measure | Value |
---|---|
Filename | H55FTAFXY_n01_p6d8.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 448860 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3227 | 0.718932406541015 | No Hit |
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1786 | 0.3978968943545872 | No Hit |
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1437 | 0.3201443657265071 | No Hit |
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 893 | 0.1989484471772936 | No Hit |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 824 | 0.18357617074366173 | No Hit |
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 697 | 0.1552822706411799 | No Hit |
TTGTAGACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTT | 629 | 0.14013278082252817 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG | 571 | 0.1272111571536782 | No Hit |
TTCTAGACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTT | 478 | 0.10649200196052222 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TTCTAGA | 125 | 0.0 | 69.6 | 1 |
ATTCTAC | 35 | 0.003565223 | 62.142857 | 3 |
TGTAGAC | 310 | 0.0 | 44.435486 | 2 |
ACTGTAT | 100 | 2.7307578E-6 | 43.5 | 8 |
TTAGAGT | 175 | 2.4228939E-9 | 37.285713 | 4 |
TAGAGTG | 200 | 2.237357E-10 | 36.25 | 5 |
TAGACGT | 1000 | 0.0 | 36.25 | 4 |
GTATTAT | 85 | 0.002740383 | 34.11765 | 1 |
CTAGGGA | 255 | 1.8189894E-12 | 34.117645 | 3 |
TCTGTCC | 215 | 4.8567017E-10 | 33.720932 | 8 |
TCTAGGG | 175 | 1.0070835E-7 | 33.142857 | 2 |
GATCTTT | 2260 | 0.0 | 33.042034 | 6 |
TACTGTA | 110 | 2.4340206E-4 | 32.954548 | 7 |
GGACCGT | 110 | 2.4340206E-4 | 32.954548 | 6 |
CCGATCT | 10950 | 0.0 | 32.906395 | 4 |
CGATCTT | 3460 | 0.0 | 32.8974 | 5 |
CTTCCGA | 11055 | 0.0 | 32.856174 | 1 |
TTCCGAT | 11050 | 0.0 | 32.674206 | 2 |
CTAGACG | 445 | 0.0 | 32.58427 | 3 |
ATCTTAC | 245 | 5.4569682E-11 | 32.55102 | 7 |