FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448860
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32270.718932406541015No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17860.3978968943545872No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14370.3201443657265071No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8930.1989484471772936No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8240.18357617074366173No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6970.1552822706411799No Hit
TTGTAGACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTT6290.14013278082252817Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG5710.1272111571536782No Hit
TTCTAGACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTT4780.10649200196052222Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTAGA1250.069.61
ATTCTAC350.00356522362.1428573
TGTAGAC3100.044.4354862
ACTGTAT1002.7307578E-643.58
TTAGAGT1752.4228939E-937.2857134
TAGAGTG2002.237357E-1036.255
TAGACGT10000.036.254
GTATTAT850.00274038334.117651
CTAGGGA2551.8189894E-1234.1176453
TCTGTCC2154.8567017E-1033.7209328
TCTAGGG1751.0070835E-733.1428572
GATCTTT22600.033.0420346
TACTGTA1102.4340206E-432.9545487
GGACCGT1102.4340206E-432.9545486
CCGATCT109500.032.9063954
CGATCTT34600.032.89745
CTTCCGA110550.032.8561741
TTCCGAT110500.032.6742062
CTAGACG4450.032.584273
ATCTTAC2455.4569682E-1132.551027