FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188126
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23891.2698935819610262No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10130.538468898504194No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9060.4815921244272455No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5440.28916789811084065No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4650.24717476584842074No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3410.18126149495550853No Hit
TTGTAGACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTT2860.1520257699626846Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC2280.12119536906116113No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA2180.11587978269882951No Hit
TTCTAGACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTT2060.10950107906403156Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA1980.10524860997416624No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC451.19134915E-464.444453
CCTACAA350.003562004562.142857145
TGTAGAC606.7842047E-660.4166682
TCTAGAC2400.054.3752
ATAATCC553.212277E-452.7272724
TTCTAGA1850.050.945951
CTAGACG2650.049.2452853
CTCCTAA450.00962706648.3333361
TGTAGAA604.9347803E-448.3333322
ATCTTGC1801.8189894E-1244.3055577
ATCTGTG1151.4044053E-744.1304327
TAGACGT5150.040.8252454
TACACAA1157.111861E-637.826084145
ACCATAC800.002032421336.251
CGATCTT14800.034.2905435
GATCTTT11250.034.1555566
CCGATCT45750.033.7540974
CTTCCGA46850.033.5805781
TTCCGAT46700.033.533192
TCATAAG1551.4566449E-632.7419362
Produced by FastQC (version 0.11.2)