Basic Statistics
Measure | Value |
---|---|
Filename | H55FTAFXY_n01_p6d4.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 136138 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1373 | 1.0085354566689682 | No Hit |
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 618 | 0.4539511378160396 | No Hit |
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 569 | 0.4179582482481012 | No Hit |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 430 | 0.3158559696778269 | No Hit |
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 284 | 0.20861184974070426 | No Hit |
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 213 | 0.1564588873055282 | No Hit |
TTGTAGACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTT | 174 | 0.127811485404516 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTATGCT | 10 | 0.007084181 | 145.0 | 9 |
ACTGTGC | 20 | 3.858806E-4 | 108.75 | 8 |
CATTGGA | 45 | 1.19016986E-4 | 64.44444 | 2 |
CTACAGG | 50 | 2.0040516E-4 | 58.0 | 1 |
TATATCT | 40 | 0.006039704 | 54.375 | 4 |
CCCCTGA | 70 | 1.688188E-5 | 51.785713 | 1 |
AATCGGA | 135 | 1.8735591E-10 | 48.333336 | 1 |
ATCTATG | 45 | 0.00962139 | 48.333332 | 7 |
ACATTCC | 45 | 0.00962139 | 48.333332 | 7 |
GTATAGC | 45 | 0.00962139 | 48.333332 | 3 |
TGTAGAC | 90 | 1.3150893E-6 | 48.333332 | 2 |
ATTATGG | 60 | 4.929936E-4 | 48.333332 | 1 |
TCTGACC | 95 | 1.9085946E-6 | 45.789474 | 8 |
CTGTCCA | 65 | 7.314179E-4 | 44.615387 | 9 |
GAACTAT | 65 | 7.314179E-4 | 44.615387 | 6 |
ATCTGTG | 115 | 1.4005673E-7 | 44.130436 | 7 |
ATAGGGA | 85 | 5.3171123E-5 | 42.64706 | 3 |
TGCAGAC | 90 | 7.4483694E-5 | 40.27778 | 2 |
TTGTAGA | 115 | 7.0973783E-6 | 37.826088 | 1 |
ATCTTGC | 125 | 1.2570919E-5 | 34.8 | 7 |