FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136138
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13731.0085354566689682No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6180.4539511378160396No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5690.4179582482481012No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4300.3158559696778269No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2840.20861184974070426No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.1564588873055282No Hit
TTGTAGACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTT1740.127811485404516Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCT100.007084181145.09
ACTGTGC203.858806E-4108.758
CATTGGA451.19016986E-464.444442
CTACAGG502.0040516E-458.01
TATATCT400.00603970454.3754
CCCCTGA701.688188E-551.7857131
AATCGGA1351.8735591E-1048.3333361
ATCTATG450.0096213948.3333327
ACATTCC450.0096213948.3333327
GTATAGC450.0096213948.3333323
TGTAGAC901.3150893E-648.3333322
ATTATGG604.929936E-448.3333321
TCTGACC951.9085946E-645.7894748
CTGTCCA657.314179E-444.6153879
GAACTAT657.314179E-444.6153876
ATCTGTG1151.4005673E-744.1304367
ATAGGGA855.3171123E-542.647063
TGCAGAC907.4483694E-540.277782
TTGTAGA1157.0973783E-637.8260881
ATCTTGC1251.2570919E-534.87