FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231808
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA25980.21090949238842419No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA20640.16755858055800904No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC20450.16601613238426766No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC19480.15814152854990388No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT18280.14839975061048474No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT17090.1387391541538941No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA14840.12047332051748323No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT14680.11917441679222736No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG13730.11146217592352053No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA13340.10829609809320934No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT13240.10748428326492439No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT13230.10740310178209592No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA12680.1029381202265288No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA12360.10034031277601704No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA45350.042.5281521
CGATCTG22850.040.2953035
TTCCGAT48100.040.2441832
CCGATCT48700.040.1949734
TATAGTA2302.7284841E-1134.6738242
ATAATTC6500.032.3460733
TCCGATC67850.028.8503323
TATAATT5950.026.8066542
GGGCGAC6050.026.363577
ATACTCC10200.026.2989544
TCCCCCC10000.026.0999348
CCATACT10350.025.917812
TAAGGTA8400.025.892795
TAGGACA6500.025.6537844
GCGCCAT6100.024.9589526
CATACTC11100.024.8197573
ATAGTAT3251.4861143E-924.53843
GATCTGG12150.024.464966
ACCATAC10900.023.2816491
CCTATAA17500.023.2018261
Produced by FastQC (version 0.11.2)