FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842820
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA17700.21000925464511994No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC15450.18331316295294367No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA14730.1747704136114473No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC14710.17453311501862795No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT13410.15910870648537054No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT11500.13644669087112316No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT11260.133599107757291No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA11000.1305142260506395No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT9620.11414062314610474No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA9440.11200493581073065No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA9040.10725896395434374No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA8790.10429273154410193No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT8740.10369948506205359No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG8720.10346218646923423No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT8520.1010892005410408No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG8450.10025865546617307No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA24800.050.8669321
CCGATCT25600.049.843754
TTCCGAT26200.048.7022932
CGATCTG12750.045.4901965
CGGGCCG2300.037.826088145
TCCGATC38050.033.725363
GTACTAT1751.0088661E-733.1428571
GGGCGAC5650.032.0796477
CTGTCTG3700.031.351353145
CCTATAA12150.028.0452671
CCATACT6800.027.7205892
TCGCGCG4750.027.4736849
GGCGACC6600.027.4621228
CTATAAG13000.027.3269232
TATAAGC13600.027.1875023
ACTGTGT2156.122664E-726.9767468
CGATCTA5150.026.7475725
CCTTATA2456.435948E-826.6326522
TCCCCCC6600.026.3636388
GCGACCC7200.026.1805559
Produced by FastQC (version 0.11.2)