FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6d11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6d11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360112
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19620.5448304971786555No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7780.2160438974541254No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6730.18688630203936551No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5340.14828719953792155No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4100.11385346781001467No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC3710.10302350379881814No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG3700.10274581241391566No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATGC803.7298858E-545.3124543
ACTGACA750.001482131738.666638
GTCTAGG1157.12325E-637.8313031
TTCTAGA1458.6685213E-735.0048261
TCTATAC850.002739679534.1176153
GCATATC1102.4330843E-432.954517
TGTAGAC2455.4569682E-1132.550992
CTAACGT2900.032.499973
GATCTTT12250.031.9591546
CCTAAAC1153.1581303E-431.5217083
CCTAACG3000.031.4166362
CCCTAAC3250.031.2350751
CTATACA950.00472334530.5262854
TCTTTTT11850.029.3670628
ATCTTTT12150.029.2386557
AACGTTG3251.8189894E-1228.9999715
ATCTCGC3750.028.9999717
TAAGCAT1504.3910193E-528.9999714
CGATCTT21700.028.732695
ATCTAGC2553.0104275E-928.4313457