FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6d10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6d10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300419
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15530.5169446672813637No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8810.2932570842722997No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6460.21503300390454663No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4850.1614411871419584No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4250.14146908151614912No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3330.11084518622324153No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG3300.10984658094195107No Hit
TTGTAGACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTT3190.10618502824388604Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTAGA1400.072.500011
TGTAGAC1301.8189894E-1255.7692342
TCTAGAC2950.049.1525422
CTAGACG3300.046.1363643
TCTGTGC1303.686855E-739.0384648
CTACACT1157.126051E-637.8260884
TAGACGT7550.034.5695344
CTACTAT850.002738947334.1176451
CCTATGC850.002738947334.1176453
GATAGAC850.002738947334.1176452
TACACTG1301.6509459E-533.461545
ATCTGTG1956.9576345E-933.461547
TCTACAC2404.3655746E-1133.2291683
GGACCGT1352.137248E-532.222226
CCGATCT77750.030.5852094
TCTGACC1902.0684092E-730.5263148
ACTACGC950.00472208930.5263148
CTTCCGA78900.030.1394161
TCCGATC79600.029.965453
TTCCGAT79200.029.842172
Produced by FastQC (version 0.11.2)