H55FTAFXY_n01_p6c9.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H55FTAFXY_n01_p6c9.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	405121
Sequences flagged as poor quality	0
Sequence length	151
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA	1303	0.32163230244791063	No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC	1079	0.26634017984750236	No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA	993	0.24511195420627419	No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT	927	0.22882052522579674	No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT	883	0.2179595725721451	No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC	861	0.2125290962453193	No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT	843	0.20808597925064365	No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG	795	0.19623766726484188	No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA	711	0.17550312128968876	No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT	708	0.17476260179057615	No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC	704	0.17377524245842602	No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT	681	0.16809792629856266	No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA	642	0.15847117281009873	No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT	630	0.15550909481364825	No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA	628	0.1550154151475732	No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA	627	0.15476857531453567	No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT	591	0.14588234132518432	No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA	575	0.14193290399658373	No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT	570	0.14069870483139604	No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG	548	0.13526822850457024	No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT	547	0.1350213886715327	No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG	539	0.13304667000723241	No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC	517	0.1276161936804066	No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA	509	0.1256414750161063	No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG	505	0.12465411568395617	No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG	502	0.12391359618484354	No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG	498	0.12292623685269338	No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC	497	0.12267939701965586	No Hit
TCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGT	491	0.12119835802143063	No Hit
GATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAA	490	0.12095151818839311	No Hit
TATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAA	481	0.11872995969105526	No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTAATAAACA	479	0.1182362800249802	No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGAC	463	0.11428684269637959	No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG	461	0.11379316303030453	No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCC	451	0.11132476469992915	No Hit
TCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGA	444	0.1095968858686664	No Hit
GTGTAAGCCTGTGAAGGTGTGTTGTAAAGCATGCTGGAGGTATCACAAGA	435	0.10737532737132857	No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA	429	0.10589428837310334	No Hit
TCATTATGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT	429	0.10589428837310334	No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCT	418	0.10317905020969043	No Hit
CAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAGAGC	413	0.10194485104450274	No Hit
TAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCT	406	0.10021697221324	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGACC	85	1.6916601E-10	68.235176	8
ATCTGAC	185	0.0	62.702595	7
CTTCCGA	2235	0.0	56.456795	1
CGATCTG	955	0.0	54.659595	5
CCGATCT	2255	0.0	54.656227	4
TTCCGAT	2405	0.0	52.453136	2
CGATCTT	445	0.0	52.134743	5
CACAGTT	45	0.009635095	48.333252	7
CGGGCCG	140	2.7102942E-10	46.607063	145
GGCAGAC	150	5.347829E-10	43.499924	7
TCTCAGC	85	5.3359006E-5	42.646984	8
AGTGCAC	155	7.385097E-10	42.096706	8
ATAACGC	155	7.385097E-10	42.096706	9
CGATCTC	855	0.0	41.549637	5
TCCGATC	3035	0.0	40.848366	3
GTGCACA	165	1.3624231E-9	39.545387	9
ACACATA	95	1.02781865E-4	38.157825	145
GAAGTGC	195	1.6916601E-10	37.17942	6
ATCTCCC	235	0.0	37.021217	7
TTCAGCC	275	0.0	36.909027	3

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content