FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445250
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA6250.1403705783267827No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC6090.13677709152161707No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5410.1215047725996631No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA4810.10802919708029197No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT4720.10600786075238629No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG7900.065.1582265
CCGATCT18150.060.7162554
TTCCGAT18550.059.407012
CTTCCGA19450.056.6580921
TCCGATC23350.048.126343
CGATCTA3900.046.474365
AGACCGC1151.4102261E-744.130432145
TCTGCGC907.475746E-540.2777758
GATCTGC5900.035.6355936
TCTTCCG11000.034.2727285
CGATCTT5550.033.9639635
GGCACTA900.00362608732.222227
GGGCGCT900.00362608732.222229
GATCTGG4750.032.052636
GTGCTCT12550.031.7729071
GTATAGC1153.159304E-431.5217361
TCGCGCG1402.7432834E-531.0714289
TACGAGT2151.8057108E-830.3488394
CGATCTC7700.030.1298715
TTATACT2651.382432E-1030.0943414
Produced by FastQC (version 0.11.2)