FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100350
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3110.30991529646238164No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.20627802690582958No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.14449427005480817No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.12057797708021924No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG1190.11858495266567017No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG1150.114598903836572No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC1110.11061285500747384No Hit
TTGTAGACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTT1090.10861983059292477Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA1070.1066268061783757No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060.10563029397110116No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC1020.10164424514200299No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA300.001930653672.51
CTGACCC300.001930653672.59
ACTGGGT300.001930653672.53
TATAATG350.003557162862.1428573
ATCTGAC1102.5465852E-1159.3181847
ATCTTGC753.726982E-758.07
CCTATAA753.726982E-758.01
TCTGACC651.0865311E-555.7692348
ATCTGTG400.006035097454.3757
CTATAAG858.8439083E-751.1764682
GGATTCT450.00961407648.3333361
TATAAGC951.9035433E-645.7894743
ATAAGCA1002.7095102E-643.54
TAGACGT1701.7953425E-938.382354
CTAGACG951.02229424E-438.1578943
GATCTAG951.02229424E-438.1578946
CGATCTT6100.038.0327875
TAAGCAA1157.078752E-637.8260845
CCGATCT21700.036.7511524
CGATCTG7100.035.7394335