Basic Statistics
Measure | Value |
---|---|
Filename | H55FTAFXY_n01_p6c7.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 100350 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 311 | 0.30991529646238164 | No Hit |
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 207 | 0.20627802690582958 | No Hit |
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 145 | 0.14449427005480817 | No Hit |
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 121 | 0.12057797708021924 | No Hit |
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG | 119 | 0.11858495266567017 | No Hit |
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG | 115 | 0.114598903836572 | No Hit |
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC | 111 | 0.11061285500747384 | No Hit |
TTGTAGACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTT | 109 | 0.10861983059292477 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA | 107 | 0.1066268061783757 | No Hit |
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 106 | 0.10563029397110116 | No Hit |
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC | 102 | 0.10164424514200299 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTACAAA | 30 | 0.0019306536 | 72.5 | 1 |
CTGACCC | 30 | 0.0019306536 | 72.5 | 9 |
ACTGGGT | 30 | 0.0019306536 | 72.5 | 3 |
TATAATG | 35 | 0.0035571628 | 62.142857 | 3 |
ATCTGAC | 110 | 2.5465852E-11 | 59.318184 | 7 |
ATCTTGC | 75 | 3.726982E-7 | 58.0 | 7 |
CCTATAA | 75 | 3.726982E-7 | 58.0 | 1 |
TCTGACC | 65 | 1.0865311E-5 | 55.769234 | 8 |
ATCTGTG | 40 | 0.0060350974 | 54.375 | 7 |
CTATAAG | 85 | 8.8439083E-7 | 51.176468 | 2 |
GGATTCT | 45 | 0.009614076 | 48.333336 | 1 |
TATAAGC | 95 | 1.9035433E-6 | 45.789474 | 3 |
ATAAGCA | 100 | 2.7095102E-6 | 43.5 | 4 |
TAGACGT | 170 | 1.7953425E-9 | 38.38235 | 4 |
CTAGACG | 95 | 1.02229424E-4 | 38.157894 | 3 |
GATCTAG | 95 | 1.02229424E-4 | 38.157894 | 6 |
CGATCTT | 610 | 0.0 | 38.032787 | 5 |
TAAGCAA | 115 | 7.078752E-6 | 37.826084 | 5 |
CCGATCT | 2170 | 0.0 | 36.751152 | 4 |
CGATCTG | 710 | 0.0 | 35.739433 | 5 |