FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567151
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA42910.273808969269713No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC31880.20342647262452693No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT29930.1909835108422864No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT29810.19021779011722545No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC29400.18760157763993388No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA29180.18619775631065547No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG27140.17318050398461923No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA24240.15467558646231283No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT23400.14931554138688613No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA22870.1459336081845336No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT22390.14287072528428976No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA21330.13610685887958468No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA19270.12296198643270495No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT18760.11970767335119589No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC18360.11715527093432605No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG17450.11134855543594716No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT17350.11071045483172967No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG17220.10988092404624698No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA17160.10949806368371649No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT17100.10911520332118603No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA16980.10834948259612508No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA16950.10815805241485985No Hit
CCTATGATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGA16690.10649899084389441No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG16380.10452087897082031No Hit
GTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC15870.10126656588931124No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAC10900.047.2246557
CTTCCGA45350.047.1668741
CCGATCT46150.046.5004274
CGATCTG19800.045.7700925
TTCCGAT47400.045.4271052
GCGACCC14700.033.5373349
ATACTCC17100.033.0700954
GGCGACC15400.032.9544688
CATACTC17650.032.8611073
ACCATAC17200.030.77421
TAGAGTG14150.030.7419745
TCCCCCC17100.030.1022668
CCATACT17600.030.070952
CTCCCCC17650.029.5749977
TCCGATC76150.028.6571853
TAATACT4700.027.7658884
GTGCTCA14400.027.6909059
ACTCCCC19300.027.4222156
CGATCTA10700.026.425175
GGGGCGA21500.026.3022616
Produced by FastQC (version 0.11.2)