FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6c4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6c4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95560
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3660.3830054416073671No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.19778149853495186No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.12766848053578903No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG1170.1224361657597321No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.11301799916282965No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC970.1015069066555044No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA960.100460443700293No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGT300.0019303772.53
TTCTAGA502.2902914E-672.51
CTAAGGA604.9225095E-448.3333322
ATCTGGC604.9225095E-448.3333327
TCTATGT450.009612679548.3333321
TAAGGAG803.709193E-545.31253
TCTGTGC700.001051845741.428578
ATCTCGA750.00147667438.6666647
TTCAGGG951.021927E-438.157892
CAGGCCG800.002027426636.254
GGAGTGT800.002027426636.256
GCTAAGG800.002027426636.251
ATCTCGT1051.8412291E-434.523817
GAATAGG1051.8412291E-434.523811
GTGTGTA850.002729650834.1176459
TCTCGTC850.002729650834.1176458
ATCGCTG850.002729650834.117645145
TCTAGAC1501.1197117E-633.8333322
CTAGACG1301.6392352E-533.461543
CCTACCT900.00361195732.222222