FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005353
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA23970.238423717838411No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC19210.19107716394142157No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT17610.17516235590882007No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA17230.17138258900107722No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC17020.1692937704467983No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG16000.15914808032601485No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT15840.1575565995227547No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA15140.15059387100849153No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT13430.1335849199236487No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA13410.1333859848232412No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT13370.13298811462242613No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA12170.12105200859797503No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA11030.10971270787474648No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT10760.10702708401924498No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT10540.10483879791476228No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG10130.10076062835640814No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC10120.10066116080620438No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA37250.054.6912731
CCGATCT37000.054.4729734
TTCCGAT38000.053.4210552
CGATCTG15700.050.3343935
TCCGATC55400.036.6425973
CGATCTA7950.036.4779855
CGATCTC17450.034.484245
TGTATAC2507.094059E-1131.93
GGGCGAC6900.030.4710167
GCGACCC7500.029.0000029
GTATATA4300.028.662791
ACCATAC9200.028.3695661
CGATCTT12800.028.3203125
CCATACT10150.026.4285722
GTACTAA2756.7648216E-926.3636361
TCCCCCC10050.025.9701488
CTCCCCC10350.025.2173927
CATACTC10750.024.279073
GTGTATA2701.6488957E-724.1666661
ATCTGAC6000.024.1666667
Produced by FastQC (version 0.11.2)