FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134189
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA23890.21063508815550142No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC22420.19767428532634332No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC20540.18109856470129757No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT19700.17369239165606437No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA17660.15570597140335515No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA17410.153501753235131No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT15210.13410463335475833No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG15100.1331347773607397No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA14660.12925535338466518No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT14400.12696296648971203No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA13900.12255453015326369No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT13720.12096749307214229No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG11410.10060051719775098No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA11380.10033601101756409No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA49600.047.2168351
TTCCGAT51300.045.7894442
CCGATCT51250.045.6926574
CGATCTG23450.044.211065
GGGCGAC8400.036.249987
TCCGATC69600.034.1666453
GGCGACC9600.031.718738
GCGACCC10000.030.4499829
TACACCG2951.4551915E-1129.491515
TAGAGTG8950.029.1619955
GTCTATA2553.0213414E-928.4338631
CGATCTT17450.027.0057145
CCTATAA17650.025.8804531
CGATCTC22200.025.1463815
ATCTGAC8150.024.907967
CGATCTA11800.023.9618515
CTATAAG18800.023.909562
CTACACC9800.022.9336594
TATAAGC19600.022.9336593
GATCTGG11400.022.8947226
Produced by FastQC (version 0.11.2)