FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614699
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA11000.178949371969045No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC10150.16512146595325516No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC8860.14413558505870352No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT8500.13827906015789843No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT8370.1361642039437188No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA8280.13470007271851753No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA7880.12819282282873407No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA6920.11257542309325377No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT6860.11159933560978626No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT6750.10980984189009581No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG6640.10802034817040534No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA6320.10281454825857858No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA24200.044.3495941
CGATCTG12200.043.9753385
CCGATCT25350.043.185334
TTCCGAT25300.042.9841162
GGGCGAC3800.034.342057
GCGCCAT3100.032.7418826
TCCGATC35350.031.5841063
TAAGGTG6500.031.2307155
CCTAGTG950.004725932630.5262642
TAGAGTG4600.029.9456025
GCGACCC5100.029.852899
GTATATG2104.970643E-727.6257441
GTATAGT1050.007697067727.6257441
AAGCGCC4000.027.1874524
CGATCTA5950.026.8066795
ATAAGGT7350.026.6326084
AGCGCCA3850.026.3635925
ATCTGAC3057.0940587E-1026.14757
AGGTGCC6950.026.0790927
TACAGGG2559.4738425E-825.5881922
Produced by FastQC (version 0.11.2)