FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6c12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6c12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234755
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA5210.2219335051436604No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4600.19594896807309747No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT4450.1895593278098443No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC4450.1895593278098443No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA4310.18359566356414134No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT3740.15931503056377927No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT3600.1533513663180763No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA3540.150795510212775No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG3490.14866563012502396No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA3130.13333049349321635No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT3120.13290451747566612No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT3050.12992268535281462No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA3040.12949670933526441No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA2780.11842133287895892No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT2760.1175693808438585No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC2720.11586547677365765No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG2670.11373559668590658No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA2590.11032778854550489No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT2520.1073459564226534No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC2360.10053034014185001No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA6100.055.8606571
TTCCGAT6300.054.0873032
CCGATCT6550.052.02294
ATATAAG1004.6826244E-850.751
GTACGGC450.0096300248.3333323
CGATCTG3100.042.0967755
GTATAGA700.00105498841.428571
TCCTTAC1303.6822712E-739.038461
CCTTACA750.001481072438.6666642
TATTGTA750.001481072438.6666642
CGATCTA1354.9497976E-737.5925945
TCCGATC10400.032.7644233
CATGGGG950.00472000730.5263183
GTTTATA950.00472000730.5263181
CGATCTC2951.4551915E-1129.4915265
CCTTACT1000.006065119529.02
GATCTAG1000.006065119529.06
AGGTGCC2752.1100277E-1029.07
TCCTAAG2705.516995E-926.8518522
CCATACT2456.394475E-826.6326522
Produced by FastQC (version 0.11.2)