FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6c11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6c11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67682
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2040.30140953281522415No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG1290.1905972045743329No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT1250.18468721373481872No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC1150.16991223663603322No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA1030.15218226411749064No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA1010.14922726869773353No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG990.14627227327797643No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA950.14036228243846222No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC940.13888478472858368No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.12854230075943382No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT860.12706480304955528No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGG760.11228982595076976No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT750.11081232824089124No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA720.10637983511125558No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA710.10490233740137703No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT680.10046984427174138No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTAA300.00192792372.58
AGACGCG350.003552150462.142857145
GATCTGT1400.056.9642876
AAGCGTC400.00602662554.3749969
TCTAGCT400.00602662554.3749963
ATCTGAC400.00602662554.3749967
CGATCTG4350.053.3333325
GAAGCGT604.912239E-448.3333328
TCTGTCC450.0096006348.3333328
CTTTGGG450.0096006348.3333322
TCTGGAG450.0096006348.3333322
GATCTCG1402.6375346E-1046.6071436
CCGATCT12650.045.276684
AGGGTAG657.287999E-444.6153835
TTCCGAT12850.044.5719872
CTTCCGA13400.042.7425351
TCCGATC13550.042.269373
CGATCTA1901.8189894E-1241.9736865
ATTTAGC700.001049664241.428572
GCTTTGG700.001049664241.428571