FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6c10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6c10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812882
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA16590.20408866231507156No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC15530.1910486392858988No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC14610.1797308834492583No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA13950.1716116238273206No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT13830.17013539480515005No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT12520.15401989464645546No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG11910.14651573045042207No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA11260.1385194899136652No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT10810.13298363108052583No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT10410.12806286767329084No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA9890.12166587524388535No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT9310.11453076830339459No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT9030.11108623391833009No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA8770.10788773770362733No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA8530.10493527965928634No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC8330.10247489795566884No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC8140.10013753533723221No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT22750.061.186762
CCGATCT22900.060.7859734
CTTCCGA23150.060.136941
CGATCTG13650.054.175785
GGGCGAC6950.044.8560757
TCCGATC35500.039.415463
GGCGACC8300.037.5602078
TATAATA2206.220944E-1032.9545172
CGGGCCG2653.6379788E-1232.830162145
TAGAGTG5850.032.2221955
GCGACCC9450.032.2221959
CGATCTC10050.031.7412665
ATACTCC8300.031.4457574
CTCCCCC8100.031.3271337
TCCCCCC8150.031.1349458
ATAATAC2351.2623786E-930.8510383
CGATCTA6400.030.585915
ACACATA4800.030.208305145
TTAGAGT7050.029.8226684
CATACTC8700.029.166643
Produced by FastQC (version 0.11.2)