FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274392
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5410.1971631826000758No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG5040.18367882445552347No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT4630.16873669786291146No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG4400.16035452928656813No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA4090.14905682381410537No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA3880.1414035394617919No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3660.1333858129974635No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA3430.12500364442112014No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC3250.11844368640485145No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT2770.10095046502813493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAAC951.915756E-645.7894742
TACGCCC657.3272426E-444.6153834
ACTAACG855.3313255E-542.6470573
CGATCTG15950.041.818185
TCTATAC700.001055299741.428573
CCGATCT41400.040.803144
CTTCCGA41750.040.461081
TTCCGAT42550.039.5299642
ATACGCC750.001481508538.6666683
TAGCCCG951.0269414E-438.1578945
TCCGATC44950.037.7419323
TAGAGTG1209.538577E-636.255
CTAACGG1001.3888191E-436.254
CGATCTT10550.035.7345965
GTCATAT850.002738535434.1176451
TCTGTGC2206.184564E-1032.9545448
CGATCTA6950.032.338135
ATCTAGC1352.1365755E-532.222227
ATCTGGC2506.91216E-1131.97
GATCTCA4550.031.8681346
Produced by FastQC (version 0.11.2)