FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621612
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA16810.2704259248534456No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC13940.22425564500041825No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC12390.19932047643867878No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT12380.19915960438344174No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT11900.19143774573206437No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA11770.189346409013983No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG10040.161515543457977No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT9910.15942420673989563No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA9520.1531501965856515No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT9160.14735880259711848No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT8740.14060217627716323No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA8600.13834996750384485No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA8540.13738473517242267No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA8090.13014549268675638No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC7580.12194101786966789No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT7310.11759747237826812No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA7240.11647136799160891No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA7060.11357567099734239No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG6880.11067997400307587No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT6850.11019735783736478No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC6810.10955386961641668No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG6670.10730166084309826No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG6600.10617555645643906No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT6440.10360160357264661No Hit
CCTATGATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGA6440.10360160357264661No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG12350.051.072855
CTTCCGA26950.050.0411071
TTCCGAT27450.049.9180072
CCGATCT27650.048.7703174
ATACGAC1105.261105E-639.5454333
ATCTGAC3300.039.5454337
TGTACTA800.00203602936.2499775
TCCGATC39350.034.822093
ATACGGC850.00274119834.117636
CATACTC5550.033.9639473
TATATAC2154.8567017E-1033.7209133
TACGCTG1102.4351022E-432.954535
GTATATA2008.931238E-932.6276051
CGATCTT9650.031.5543885
AGACCGC2101.4379111E-831.071411145
GGGCGCT1402.7450289E-531.071419
TAGAGTG5200.030.6730615
CCATACT5750.030.2608532
GTCAATA1255.149216E-429.0023161
GTATAAC1255.149216E-429.0023161
Produced by FastQC (version 0.11.2)