FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994389
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA31300.15694029600042922No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC23690.11878324639776894No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC23660.11863282438882283No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT23250.11657705693322616No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT23050.11557424354025217No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA20780.10419231152999742No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG20140.10098330867248065No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA20040.10048190197599365No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT65500.046.7099084
CTTCCGA65700.046.2389831
TTCCGAT67550.045.0777052
CGATCTG31450.043.1080935
TCCGATC94650.032.5541343
CGATCTA16750.028.5671545
CCTACAC12800.027.187493
GTATATA6300.026.469571
TCCCCCC14350.025.2613148
GGGCGAC10850.023.3870897
CTACACC15650.023.1629334
CGGGCCG3452.9685907E-923.115934145
CGATCTC32900.022.697565
CCTATAA26500.022.161481
CTCCCCC16650.021.7717637
CTGTCCG3355.505717E-821.6417839
AATACCG20550.021.5206745
TCCTACA15850.021.4984172
GTCTATA4751.2732926E-1121.3694861
TCGCGCG8550.021.1988229
Produced by FastQC (version 0.11.2)