FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985851
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA20590.20885509067800306No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT18770.19039388305129276No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC18670.18937953098389107No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC18620.18887235495019025No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA16350.16584656302017242No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA15470.15692026482703775No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT14710.1492111891147851No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT14660.14870401308108427No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG14510.14718248497998176No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT13160.13348873207005926No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA11990.12162081288145977No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA11650.11817201585229412No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA11340.11502752444334893No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA11300.11462178361638829No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT10920.11076724576026195No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG10300.10447826294237163No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA10090.10234812360082812No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA38050.046.303141
CCGATCT39550.045.644634
CGATCTG18200.045.411965
TTCCGAT39250.045.254652
GGGCGAC6450.039.3409777
TCCGATC54400.033.184653
TAGAGTG8250.032.515065
GCGACCC8500.032.4116749
GGCGACC8100.032.2221348
GTGCTCA8600.029.505739
CGATCTT12550.028.884385
TCCCCCC8350.027.7843518
TTAGAGT9500.027.4736064
CTATAGG1606.825813E-527.1888031
CGATCTA9200.026.7934045
AGAGTGC13500.026.314746
CCATACT9200.025.2173212
CTGCACG1751.255732E-424.8570739
CATACTC11450.024.6942563
GAGTGCT14500.024.499937
Produced by FastQC (version 0.11.2)