FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943328
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA18620.1973862749754062No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC17440.18487737033142237No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC16820.17830489501000713No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA15740.16685606703076766No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT15670.1661140133654466No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA14250.1510609247260762No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT12600.1335696597577937No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT12440.13187353709420266No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT12150.1287993147664439No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG11750.12455900810746633No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA10600.11236812646290578No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA10350.10971793480104482No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA9900.10494758980969504No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT33900.049.8303644
TTCCGAT35650.046.9775432
CTTCCGA35850.046.717941
CGATCTG15650.044.0095675
TCCGATC50200.033.7948073
CGATCTT11500.029.6304235
CGATCTC15600.029.2788375
TAGGACA4400.028.0113544
CCTATAA13300.027.8022161
GTATAGA4550.027.0893381
ATGTAGG4300.026.9781671
TAAGGTG10650.026.5492845
GGCGACC6950.026.0791288
AGGTGCC9700.025.4123617
GTAGGCA4051.8189894E-1225.061723
CTATAAG14400.024.6701282
GGGCGAC7650.024.6405147
CGATCTA8900.024.4381945
TGTAGGC4500.024.1666582
GCGACCC7700.023.5389529
Produced by FastQC (version 0.11.2)