FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598365
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA11770.19670268147368247No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC11400.19051916472387256No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT11280.18851369983204233No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC10580.17681515462969927No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA10430.17430832351491146No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA10310.17230285862308123No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT8430.14088390865107417No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT8030.13419902567830672No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT7370.12316896877324042No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA7190.12016077143549506No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA6880.11497998713160028No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG6830.11414437676000434No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT6660.11130330149657817No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA6410.10712524963859853No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA6350.1061225171926834No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA6350.1061225171926834No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG6040.10094173288878862No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAC3950.040.3797267
CCGATCT31650.040.3159334
CTTCCGA31900.040.230611
TTCCGAT32500.039.0384372
CGATCTG13550.038.5239645
CGGCATA800.002035968236.2499779
GCGACCC5150.033.786399
GGCGACC5150.032.378628
TAGACTG1153.1606038E-431.5217195
TCCGATC41100.031.399013
CGATCTA6850.029.635025
ACCATAC5400.029.5394861
CGATCTT10000.028.9999855
CGGGCCG1306.4799044E-427.8846145
CCATACT5550.027.4324152
CATACTA1358.0789276E-426.8518352
TATACTG2456.4294E-826.6326395
GTACAGG1100.009664630526.3658241
ATATACA4500.025.7777614
TAGAGTG5500.025.045445
Produced by FastQC (version 0.11.2)