FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6b12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6b12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56356
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1740.3087515082688622No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.17212009369011286No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG880.1561501880899993No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730.1295336787564767No Hit
CGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.11888707502306765No Hit
GACGTGTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.1153382071119313No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC610.10824047128965861No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.10469160337852226No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCGG259.3405176E-487.06
CAGAGTG300.001926243972.499994
ATTAGAG400.00602141354.3753
TCTCTAC400.00602141354.3758
GTACTGG400.00602141354.3751
CCTAACG553.1929617E-452.7272762
CTAACGT553.1929617E-452.7272763
CTAGAAA450.0095923648.3333324
GGTTAAG450.0095923648.3333321
GTAGACG803.6896963E-545.31253
CCCTAAC657.2775775E-444.6153831
TAGACGT1105.176342E-639.5454564
AACGTTG750.001471525338.6666645
TAACGTT750.001471525338.6666644
AATCGGA750.001471525338.6666641
TAGACAG800.002020377736.255
GATCTGA1051.8316673E-434.5238086
ACGTTGC850.002720188634.1176456
CCGGGAG900.003599473332.222229
CGTTGCC900.003599473332.222227