FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6b11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6b11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844681
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA18540.21949114517788373No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC16290.19285387027765513No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC14810.17533246278772696No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT13790.16325689816629No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA13600.16100752828582626No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT12750.15094455776796212No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT12440.14727453322615283No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA11800.13969770836564335No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG11020.13046345306689744No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT10490.12418889497928805No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA9720.11507302756898759No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA9260.1096271847004964No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT9240.10939040892360549No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA8980.1063123238240235No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA8750.10358940238977793No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT8620.10205035983998692No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC8600.10181358406309601No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT8550.1012216446208687No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA23600.056.839171
CCGATCT24750.054.1949464
TTCCGAT25150.053.621292
CGATCTG11600.046.877625
CGATCTA5350.043.3669135
CCCACGC2100.041.428432145
TAGAGTG5800.036.252035
TCCGATC38950.034.2509543
GGGCGAC4900.034.0325167
CGATCTC11300.032.723075
TTAGAGT7150.032.4493684
TCCCCCC6850.029.634948
GGCGACC6150.029.471458
AAATTAG6900.028.372831
CTCCCCC7450.028.2230557
CCATACT7750.028.0660862
CTGACAG2603.7161954E-927.8861736
GTCCGCT2654.5511115E-927.361641
ACCATAC8550.027.1376291
GTGCTCA6850.026.4597669
Produced by FastQC (version 0.11.2)