FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6b10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6b10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532282
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA8800.16532589867776856No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC7910.14860543847058516No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC7470.14033914353669671No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT7400.13902405116085081No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA7050.1324485892816214No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT6830.1283154418146772No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA6360.11948553586256909No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT6110.11478877737740523No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG5930.11140711126808721No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT5900.11084350024986755No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA5470.10276507565538569No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA5460.10257720531597914No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA17250.057.1647531
CCGATCT17950.054.526434
TTCCGAT18000.054.3749662
CGATCTG8400.050.059495
TATTAGT752.5489713E-548.3333052
TCCGATC23050.042.462013
CTATACT800.002035767536.2499774
CGATCTT6200.036.2499775
GGGCGAC3700.035.270257
GGCGACC4000.034.4374778
TAATACT1402.7442953E-531.0714074
CCTAGAT950.00472534530.5262972
CTATTAC2151.8055289E-830.3516671
GCGACCC4750.028.999989
TATACTG1255.1506615E-428.9999795
TAGACTG1555.495682E-528.0644995
ATCTAGG1306.475401E-427.8872171
TCTTCCG11000.027.6817995
GTACAGG1050.00770175727.6216241
CTCCCCC4600.026.793467
Produced by FastQC (version 0.11.2)