FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845214
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA15470.18303056977286228No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC12290.1454069620238188No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC11230.13286575944080434No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT11230.13286575944080434No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT11050.1307361212663302No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA11010.13026286833866926No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA10640.12588527875780572No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG10360.12257250826417924No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT9590.11346238940670647No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA9530.11275251001521508No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT9100.10766504104286015No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA8530.100921186823692No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA27250.047.6322481
CCGATCT28400.045.1848034
TTCCGAT29100.044.097882
CGATCTG13450.043.1226235
GGGCGAC5750.036.5651747
CAGGGGC2800.036.2499544
CGATCTA6100.033.278655
TCCGATC41050.031.6138463
GGCGACC7250.030.9999648
GCGACCC8000.030.8124649
GTATAAT2752.1282176E-1029.0051121
CATTACG1804.698877E-628.194419
GGGTACC1555.4994754E-528.0644826
CGTTATA1306.4821605E-427.8845792
CTAGGGG1606.826963E-527.1874683
CCTACAT3503.6379788E-1226.928543
TCCCCCC7000.026.928548
CTCCCCC7350.026.6326187
GTCGCGC6550.026.5648548
ATACTCC8500.026.4411434
Produced by FastQC (version 0.11.2)