FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465736
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATAGGTATGCAAAGCGAACGCGGCGAACTGAAACATCTAAGTAGCT8650.18572753663019392No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5840.12539292646477831No Hit
GTACTGGTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGT4920.10563924626827215No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC4790.10284796537093976No Hit
ATCACATACAGGCTCATCCCATTTCGCTCGCCACTACTTTGGGAATCTCG4730.10155968187986328No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG18100.055.276125
CCGATCT46050.054.4732744
CTTCCGA47600.052.716441
TTCCGAT48300.051.9357032
TCCGATC49900.050.270433
CGATCTT11950.043.681915
CGATCTA9350.041.0961655
CGATCTC15150.040.676485
CCCTAGA907.476371E-540.2776872
CACTAGG1354.951344E-737.604621
TCTAGGG1209.553029E-636.249922
GATCTGG7050.034.964466
GATCTGC11450.034.825256
GATCTCG5200.033.4614646
CCTAGAC1102.4341932E-432.9544683
GATCTTT7800.031.6024956
GATCTGA6050.031.1569546
ATCTGCT2859.094947E-1230.5262497
TCTGACC1902.0725929E-730.5262478
ATCTGAG2502.4392648E-928.9999357
Produced by FastQC (version 0.11.2)