FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564103
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG24440.4332542106672009No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT22180.39319060526180505No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC21490.38095879653183906No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT20730.36748607966984753No Hit
TCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGT20200.35809063238451133No Hit
GATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAA19920.3531269998564092No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG17540.31093612336754106No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG17280.30632703602001765No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCT16420.2910815932551325No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGG16210.28735886885905587No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG16080.28505432518529417No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA15750.2792043297057452No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT14720.26094525290594095No Hit
ATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAATGCCTCTAA14660.2598816173642048No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT14050.24906798935655367No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTT13960.24747253604394942No Hit
TTTCAAGACGGGTCGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGC13820.24499071977989836No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC13730.2433952664672941No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGT13430.23807708875861322No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT13350.23665890803629833No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC13220.23435436436253662No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC13210.23417709177224727No Hit
TCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGA13150.23311345623051108No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA13140.23293618364022173No Hit
TATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAA12900.22868164147327705No Hit
CCTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTA12800.22690891557038342No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA12590.22318619117430682No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCC12410.2199952845490983No Hit
GTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGA12250.2171589231044685No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC12180.21591801497244295No Hit
GGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTG11960.212018017986077No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC11850.21006801949289403No Hit
TAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCT11820.20953620172202594No Hit
CCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGAC11740.20811802099971105No Hit
GTCTTGAAACACGGACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTG11480.2035089336521876No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGAC11380.201736207749294No Hit
TCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTT11260.19960893666582166No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC11190.19836802853379615No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCT11060.19606348486003441No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT10600.18790894570672378No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT10490.18595894721354078No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG10390.18418622131064716No Hit
GTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAA9790.17354986589328544No Hit
AGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGC9770.17319532071270674No Hit
CGTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCA9390.16645896228171098No Hit
TTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCC9370.16610441710113225No Hit
ATACTGACGTGCAAATCGTTCGTCTGACTTGGGTATAGGGGCGAAAGACT9350.16574987192055352No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT9330.16539532673997479No Hit
GGCTTAGGCCGCCACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCG9190.16291351047592373No Hit
TCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAG9140.16202714752447692No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA9130.16184987493418757No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATT9100.16131805716331946No Hit
CTCCACAAGCGTATCAAAAGCCCGGGCTTAGGCCGCCACCGTAATCCGCG9010.1597226038507152No Hit
ATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCG9010.1597226038507152No Hit
CCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTT8950.15865896830897902No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA8930.15830442312840032No Hit
CATGTCAGACTCCTTGGTCCGTGTTTCAAGACGGGTCGAATGGGGAGCCC8900.15777260535753224No Hit
TGTCAGACTCCTTGGTCCGTGTTTCAAGACGGGTCGAATGGGGAGCCCAC8830.15653169722550667No Hit
GGCTTTACCCCGCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGC8820.15635442463521734No Hit
GTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGA8660.15351806319058753No Hit
TCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAAT8630.15298624541971945No Hit
GATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGT8600.15245442764885136No Hit
ATGGTGAACTATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAA8440.14961806620422158No Hit
AACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGG8400.14890897584306412No Hit
TCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGC8380.14855443066248541No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT8330.1476680677110386No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTA8250.14624988698872368No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAAGGCTAAAT8150.14447716108583006No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG8110.14376807072467263No Hit
GTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGG8090.1434135255440939No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA8060.1428817077732258No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA7990.14164079964120027No Hit
GTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAA7840.13898171078685984No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCG7790.13809534783541302No Hit
GGATAGCTGGAGCTCGGACGCGAGTTCTATCGGGTAAAGCCAATGATTAG7740.13720898488396624No Hit
GGCATAGTTCACCATCTTTCGGGTCCCGACAGGCATGCTCACACTCGAAC7640.13543625898107262No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG7620.13508171380049389No Hit
AACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAA7600.13472716861991516No Hit
CCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTC7430.13171353458499602No Hit
ATTCTCAAACTTTAAATAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGT7380.1308271716335492No Hit
GTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATC7310.12958626350152366No Hit
ACCTTGATCTTCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCC7300.12940899091123428No Hit
CCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCG7280.12905444573065558No Hit
GTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATT7210.12781353759863004No Hit
CTTCTCAGAAGATCAAGGTCGGTCGGCGGTGCACCCGCGAGGGATCCCGC7210.12781353759863004No Hit
CCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGG7200.12763626500834066No Hit
GATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTTGCAG7140.1265726294666045No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGT7020.12444535838313216No Hit
AACTATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGC7020.12444535838313216No Hit
ACCCGATAGAACTCGCGTCCGAGCTCCAGCTATCCTGAGGGAAACTTCGG7010.12426808579284279No Hit
GAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG7000.12409081320255344No Hit
GTTCACCATCTTTCGGGTCCCGACAGGCATGCTCACACTCGAACCCTTCT6980.12373626802197471No Hit
ACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCGGCGGACCGGATTG6780.12019081621618746No Hit
GACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAA6680.11841809031329384No Hit
ACTTAGGCCCGGTCCGTCGCTGAGGACGCTTCTCCAGACTACAATTCGAA6650.11788627254242577No Hit
CCTAACGGCGTGCCTCGGCATCAGCGTGCTCCGGGCGTCGGCCTGTGGGC6630.11753172736184705No Hit
TAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAG6630.11753172736184705No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC6580.11664536441040024No Hit
ACTTTAAATAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGA6530.11575900145895342No Hit
GAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGTAGA6530.11575900145895342No Hit
CCCAATGCCTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCT6520.11558172886866405No Hit
CCGTTAGGCGCGTGCTGCAGACCACGATCACGGCAGCGACGTCTCCACAA6510.1154044562783747No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGT6480.1148726385075066No Hit
TCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCGG6410.11363173037548108No Hit
GAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGC6380.11309991260461298No Hit
ACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCTTCCAC6340.11239082224345555No Hit
ACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGT6280.11132718670171936No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG6220.1102635511599832No Hit
AGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGTGAACTATGCCTGAGC6220.1102635511599832No Hit
CTTTAAATAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAA6210.11008627856969383No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAG6190.1097317333891151No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG6180.10955446079882576No Hit
TCTCGATTCCGTGTGACGGCTCAACAAAGCAGCCGACACGTCCTACCTAT6160.10919991561824702No Hit
ACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCG6130.10866809784737894No Hit
GAACTATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCG6120.10849082525708957No Hit
GAATAGGTCGAGGGCGTTGCGCCCCCAATGCCTCTAATCATTGGCTTTAC6110.10831355266680022No Hit
GATCAAGGTCGGTCGGCGGTGCACCCGCGAGGGATCCCGCCAATCAGCTT6050.10724991712506404No Hit
ACTTGGAGCTCTCGATTCCGTGTGACGGCTCAACAAAGCAGCCGACACGT6010.1065408267639066No Hit
GTTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGC6000.10636355417361723No Hit
GTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTT5940.10529991863188107No Hit
TCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGA5860.10388173790956616No Hit
GACTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTC5850.10370446531927681No Hit
CCTTGGTCCGTGTTTCAAGACGGGTCGAATGGGGAGCCCACAGGCCGACG5840.10352719272898744No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG5840.10352719272898744No Hit
CTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAA5820.10317264754840871No Hit
GGTCTGCAGCACGCGCCTAACGGCGTGCCTCGGCATCAGCGTGCTCCGGG5780.10246355718725125No Hit
GTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAGAGTGCTTGAAATTG5770.1022862845969619No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA5770.1022862845969619No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT5720.10139992164551509No Hit
CTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGT5700.10104537646493637No Hit
CTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGAT5670.10051355869406828No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAT203.8651837E-4108.74992145
ATAAATA1900.072.499954145
CTCCTCT406.6606925E-572.4999549
ATCTGCG903.6379788E-1272.4999547
ATCTCGC902.8194336E-1064.4444057
CTTCCGA23100.063.089971
ACACATA1051.6370905E-1162.14282145
TATGCCG350.003565706362.14282145
TCTACGT350.003565706362.142823
TGCAGGG1051.6370905E-1162.142822
TTCCGAT23400.061.9657752
CAAAGCC2250.061.222183145
CCGATCT23750.060.7473344
ATAGTTT502.0094954E-457.999974
CGATCTA3900.055.7691965
CGATCTC7200.054.3749665
TCTGTAA400.0060495454.374968
CGATCTG10750.053.9534575
TCTCCTC553.217807E-452.7272388
GGGCAAC553.217807E-452.7272386
Produced by FastQC (version 0.11.2)