FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410096
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA8730.21287698490109636No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC7810.1904432132963989No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC7320.17849479146346223No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA7160.1745932659669931No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT6900.1682532870352308No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA6870.1675217510046428No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT5380.13118879481877416No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT5250.12801880535289298No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5220.12728726932230502No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG5000.12192267176465998No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA4830.11777730092466154No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA4790.11680191955054425No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA4780.11655807420701494No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT4570.11143732199289923No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA4420.10777964183995943No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA4290.10460965237407825No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG4170.10168350825172642No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT4150.10119581756466778No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAGG753.7559948E-757.9993131
GGGCGAC4200.039.7019167
GCGACCC4150.038.433289
CGATCTG12600.037.4003565
ATACTCC3550.036.7601284
GGCGACC4150.036.6863148
TCCCCCC3500.035.213878
TACTCCC4400.034.6018685
CATACTC4200.034.5234033
CCGATCT27550.034.2101254
CGGGCCG850.002740266734.117245145
CCATACT3850.033.8957062
CTTCCGA28750.033.2865681
TTCCGAT28900.032.6120722
TAGACGT2755.456968E-1231.6359924
ACCATAC3750.030.932971
CTCCCCC4150.029.6984447
TCCTAGG1000.006070685628.9996592
GTTAGTA1306.476823E-427.8842873
ATAACGC1606.819024E-527.1871819
Produced by FastQC (version 0.11.2)