FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1759145
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA50020.28434267783497097No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC39510.22459774492722315No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC39130.22243760463179557No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA37310.21209166953264227No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT36280.2062365524160885No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT33900.19270725267104188No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA32010.18196339699115197No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT30540.173607064795682No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT25350.1441040960239207No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG25100.14268295109271834No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA25060.14245556790372596No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA24690.14035227340554643No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA23590.134099235708256No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT22420.12744827743022888No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT22350.12705035684949223No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA22330.12693666525499603No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG21830.12409437539259129No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC21670.12318484263662177No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT20180.11471481884665562No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA20070.11408951507692658No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG19590.11136091680901802No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA19360.11005346347231183No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC19340.10993977187781563No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG19030.10817755216312469No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT18460.10493734171998327No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG32000.047.3515325
CCGATCT76250.045.8294834
TTCCGAT78650.044.523182
CTTCCGA79250.044.2813571
CATACTA6100.034.4671942
TCCGATC107750.032.9025343
CGATCTA16650.031.3513365
ATCTGCC6850.029.635027
CATACTC18900.029.537023
CCTATAA31850.029.3666921
CCATACT18400.029.1575932
ATACTCC19500.028.628194
CGATCTC34150.028.2357085
CTATAAG33250.028.1278022
TCCCCCC18900.027.6190328
ACCATAC19650.027.305111
ACTACCG7250.026.9999855
TATAAGC36350.026.9257053
GCGCCAT8100.026.8518356
CTCCCCC20650.025.6295247
Produced by FastQC (version 0.11.2)