FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6a12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6a12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435244
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA8780.20172592844473447No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC7560.1736956741505914No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC6880.15807225372434772No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT6720.15439615480052568No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA6190.1422190771153652No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT6020.13831322200880428No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA5750.13210980507485456No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT5560.1277444376028159No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG5490.12613614432364376No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT5350.12291955776529946No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA5340.1226898015825606No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA4820.11074248008013894No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA4590.10545808787714478No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA4380.10063320803962836No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA13900.056.3367421
CCGATCT13950.056.128374
TTCCGAT14050.055.7288742
CGATCTG6500.049.076345
CGATCTA3000.038.666215
TCCGATC21350.037.3531883
CGGGCCG1251.2635524E-534.799587145
TATATAC1956.9740054E-933.461143
TAGTGTA900.003626208832.2218369
TAAGGTA2755.456968E-1231.6359865
TAAGGTG5550.030.0446875
CTGTAAG1504.389984E-529.0029891
CGATCTT7000.028.9996555
CATAAGG6050.028.7599893
AGGTGCC5600.028.4818047
TCCCCCC3351.8189894E-1228.1339938
GCCTATA1050.00769987427.6218911
CTAACCG1050.007704193727.6187219
ACTGTAC1358.075899E-426.8515343
ATACTCC3800.026.7102094
Produced by FastQC (version 0.11.2)