FastQCFastQC Report
Wed 12 Sep 2018
H55FTAFXY_n01_p6a10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55FTAFXY_n01_p6a10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886964
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA23020.25953702743290596No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC19670.2217677380367185No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC19480.2196255992351437No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA18290.2062090456884383No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT17180.19369444532134336No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT15270.17216031315814398No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA14750.16629761749067606No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT14600.16460645527890647No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG14080.15874375961143855No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT13500.15220459905926284No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA11930.13450376790940782No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT11470.12931753712664776No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA11350.12796460735723209No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA10420.11747940164426064No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA10240.11545000699013715No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC10190.11488628625288061No Hit
ATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACA10080.11364610063091625No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA9890.11150396182934144No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG9850.1110529852395362No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT9650.1087981022905101No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG9600.10823438155325357No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT9590.10812163740580227No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT9370.10564126616187354No Hit
GTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC8930.10068052367401609No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT23250.059.8709452
CTTCCGA23750.058.0032461
CCGATCT24900.055.0300984
CGATCTG13400.047.6119235
TCCGATC41000.034.3048633
CGGGCCG1801.2921191E-732.22221145
TCTTCCG13900.031.8165365
GTATATA4100.030.0626581
CGATCTA5600.029.7767735
GGGTACC2252.8163413E-828.999996
CGATCTC11950.028.5146355
TAGGACA5700.027.9824454
TGCAGGG2853.237801E-1027.9824452
CGATCTT8650.027.6589475
TAGAGTG7900.027.5316375
ACCATAC9200.026.794981
TATAATA3005.857146E-1026.5833232
GGGCGAC7250.025.999997
GGCGACC7700.025.4220688
ATACTCC10300.025.3397964
Produced by FastQC (version 0.11.2)