FastQCFastQC Report
Fri 24 Feb 2017
H55C2BGX2_n04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101454299
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22764612.243829017043428No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATAG550100.014.23185169
TTACGCG419750.013.70946469
GGATAGT419800.013.412333569
GATCTTA1081750.013.12396369
TCTTACG680300.013.07827669
GTATTAG167050.013.0504841
ATCTTAC931900.012.71990469
TACGCGT360100.012.65993469
CTTACGC521200.012.62107169
GTATATA258550.012.5011831
GATAGTG414400.012.27329969
ACGCGTG293250.012.24403169
GTATTAT296250.012.1212781
GTATAGA212950.012.0193861
CTATACT167750.011.8637024
CGAAGAG2200300.011.58426469
GTATAAA382800.011.5433861
CGATCTT1432950.011.39113669
TTATACT413950.011.3901014
AGAGGAT1116700.011.3369869
Produced by FastQC (version 0.11.2)