FastQCFastQC Report
Fri 24 Feb 2017
H55C2BGX2_n03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101454299
Sequences flagged as poor quality0
Sequence length8
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTACGC2079631220.498206783726335No Hit
AGAGGATA1657552016.337917824458085No Hit
TCTACTCT1430708214.101996801535241No Hit
ATAGAGAG1252325012.3437351826757ABI Solid3 Adapter B (100% over 8bp)
TACTCCTT97609999.621079733644407ABI Solid3 GAPDH Reverse Primer (100% over 8bp)
TATGCAGT72745517.170273780118475No Hit
CTCCTTAC72579717.15393144651268No Hit
AGATCTCG63326926.2419158797795244Illumina Single End PCR Primer 1 (100% over 8bp)
AGGCTTAG31981713.1523267436897866No Hit
GGGGGGGG9941390.9798884914674735No Hit
TGGGGGGG2259670.22272787080220227No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)