FastQCFastQC Report
Fri 24 Feb 2017
H55C2BGX2_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101454299
Sequences flagged as poor quality0
Sequence length8
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGAGGA7179218570.7630782604885No Hit
GGGGGGGG101363989.991097567979846No Hit
CGAGGCTG28782032.836945332400355No Hit
GCTACGCT23991872.364795798352517No Hit
AAGAGGCA16295991.606239475372059No Hit
GGACTCCT15328751.5109019677914288No Hit
CTCTCTAC15304161.5084782163839108No Hit
CGTACTAG13463301.3270310014167068No Hit
TAGGCATG12912651.2727553319352194No Hit
CAGAGAGG12437031.2258751105263663No Hit
TCCTGAGC11220671.105982704587018No Hit
AGGCAGAA9116320.8985641899708953No Hit
TAAGGCGA7470090.736300982179178No Hit
GTAGAGAA2887940.2846542757148221No Hit
GTAAGGAA2116960.2086614387824019No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)