FastQCFastQC Report
Fri 24 Feb 2017
H55C2BGX2_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101454299
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11195871.1035382542044867No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAGA2055050.016.40965769
GACGTAG2606900.016.20831369
CGTAGAG1592100.015.588860569
AGACGTA3329400.014.50771569
GTAGAGG1307300.014.19183769
TAGAGGA1125800.013.95909569
GTATTAG164600.013.3778751
GTATATA272250.012.8548311
GTATTAT302250.012.5495361
GAGACGT4246250.012.51427669
CTATACT162850.012.4774064
GTATAAA392100.012.2793021
TATACAG229400.012.0608565
TTATACT423050.011.7753084
TATACTG276650.011.6968615
TAATACT256900.011.6695054
GTATAGA215050.011.6197141
ATAATAC318950.011.5517093
GAGGAAT910900.011.01769169
TACACAG209400.010.9887015
Produced by FastQC (version 0.11.2)