FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_8_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_8_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303429
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5760.18983024035276785No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGCT400.002591479334.5050314
GTATAGG450.004614087330.6711391
CTAACGG500.007720590627.60402518
GTACTGT1301.8354694E-723.88811
TATTGGA1253.4947352E-622.0759372
GACTGTT953.0554103E-421.79265211
ATGGTGG800.00288934821.56920248
GTTTTAC800.002892115621.5656451
TGGACAG1154.5889712E-520.99965
GTATTGT1004.3204185E-420.7030181
CTTCTAT1258.704244E-519.3228191
GTTTGCG950.00785217318.14557860
CTAAAAC1300.002519063415.9201483
AGGCATA1350.003225454115.3406324
CCCATGA2051.4338293E-515.1485513
TATGCGG1605.741572E-415.0934625
GGTGTAG1851.023278E-414.90879968
GTGTAGA1851.023278E-414.90879969
CTTAGGT3302.6138878E-914.62643669
GCAGGAG1901.278706E-414.5236433
Produced by FastQC (version 0.11.2)