FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_sp29_7_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_sp29_7_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114562
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2690.234807353223582No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACCC150.002353188868.977283
GTCCAAG200.0073263951.77820611
CACCTGA200.007339072451.7555737
CTACTAC200.00735177151.7329646
TTACTAA200.007377217551.687864
ATCGGCA350.001342030739.4328167
TTGTAAT450.004593224730.6833811
ACAGTAT605.4025394E-428.7405343
CCTATTC500.007685839727.6150448
TAGGCTT1602.9212346E-517.2443225
TTAGGCT1452.6844296E-416.6642510
TACAGTA1300.002508655715.9178342
CTTAGGC1605.6784885E-415.10197615
TACTCCT3605.638867E-1014.35772169
CTCCTTG2403.9767638E-614.35772169
AGGCTTA1500.00645823313.80752112
ATACTCC4254.838512E-1012.97262469
ACTCCTT2953.329046E-511.6808668
GATACTC4905.5135388E-810.5485367
CGATACT5907.681592E-99.9287369
Produced by FastQC (version 0.11.2)